Long non-coding RNA NEAT1/miR-320b/MSI2 axis regulates cisplatin resistance in ovarian cancer

Introduction Ovarian cancer (OC) frequently occurs in postmenopausal women and it has higher mortality rate. Accumulating researches proved that long non-coding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) involved the progression of chemoresistance human OC. Here, study aimed to investigate partial molecular mechanism OC chemoresistance. Material methods The levels NEAT1 microRNA-320b (miR-320b) were measured by qRT-PCR. Western blot was carried out determine protein used this research. Cell viability identified via Counting Kit-8 (CCK-8). Transwell assay employed migration invasion. relationship between miR-320b or MSI2 clarified dual-luciferase reporter assay, immunoprecipitation (RIP) pull down assay. Also, a murine xenograft explore effect on cisplatin resistance vivo. Results level significantly increased resistant cell lines. Downregulation enhanced sensibility OVCAR-3/DDP HEY/DDP cells. Furthermore, target NEAT1, effects knockdown viability, IC50 cisplatin, invasion restored inhibitor miR-320. In addition, directly targeted regulate downregulation decreased Conclusions regulated through NEAT1/miR-320b/MSI2 axis OC, which might offer novel therapy for chemotherapy