Long non-coding RNA NEAT1/miR-320b/MSI2 axis regulates cisplatin resistance in ovarian cancer

نویسندگان

چکیده

Introduction Ovarian cancer (OC) frequently occurs in postmenopausal women and it has higher mortality rate. Accumulating researches proved that long non-coding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) involved the progression of chemoresistance human OC. Here, study aimed to investigate partial molecular mechanism OC chemoresistance. Material methods The levels NEAT1 microRNA-320b (miR-320b) were measured by qRT-PCR. Western blot was carried out determine protein used this research. Cell viability identified via Counting Kit-8 (CCK-8). Transwell assay employed migration invasion. relationship between miR-320b or MSI2 clarified dual-luciferase reporter assay, immunoprecipitation (RIP) pull down assay. Also, a murine xenograft explore effect on cisplatin resistance vivo. Results level significantly increased resistant cell lines. Downregulation enhanced sensibility OVCAR-3/DDP HEY/DDP cells. Furthermore, target NEAT1, effects knockdown viability, IC50 cisplatin, invasion restored inhibitor miR-320. In addition, directly targeted regulate downregulation decreased Conclusions regulated through NEAT1/miR-320b/MSI2 axis OC, which might offer novel therapy for chemotherapy

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ژورنال

عنوان ژورنال: Archives of Medical Science

سال: 2021

ISSN: ['2657-7941']

DOI: https://doi.org/10.5114/aoms/131566